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. 2008 Apr 18;190(12):4281–4290. doi: 10.1128/JB.01875-07

FIG. 2.

FIG. 2.

(A) Schematic representation of the genomic organization of the glycosylation loci BP0399 and BP0398 in B. pertussis strain Tohama I. Orthologous genes are found in other bordetellae, including B. bronchiseptica RB50 (locus tags BB4269 and BB4268), B. parapertussis hu 12822 (locus tags BPP3825 and BPP3824), and B. avium 197N (locus tags BAV2928 and BAV2927) (54, 63). BP0399 and BP0398 encode putative glycosyl transferases belonging to CAZy families 2 and 83, respectively (1). BP0397 and BP0396 encode putative proteins of as-yet-unknown function. #, characters in capital indicate imperfect heptads identified by the BvgA binding site motif, and characters in lowercase indicate a base between the heptads (19). (B) Results of semiquantitative RT-PCR analysis (boxed) and PCR controls of BP0399, BP0398, and vag8 with B. pertussis strain BP338 (wild type) and its isogenic mutant BP347 (bvgS::Tn5) after growth in SS broth and on BG agar. †, RT-PCRs using cDNA from 100 ng total RNA as a template; ‡, control PCRs using 100 to 200 ng total RNA as a template; +, control PCRs using 100 ng genomic DNA as a template; −, control PCRs without a template.