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. 2008 Apr 11;190(12):4198–4209. doi: 10.1128/JB.00153-08

FIG. 2.

FIG. 2.

(A) LPS from A. hydrophila AH-3 (wild type) (lane 1), mutants AH-901, AH-902, AH-405ΔwecA, and AH-405Δwzy (lanes 2, 3, 4, and 5, respectively), mutants AH-901 and AH-902 complemented with plasmids COS-O34 and AH-405ΔwecA, respectively (lanes 6 and 7, respectively), and mutant AH-405Δwzy complemented with pBAD33-wecA and pBAD33-wzy (lanes 8 and 9, respectively). LPS was extracted and analyzed by 12% SDS-PAGE as described by Darveau and Hancock (14). (B) SDS-Tricine-PAGE gels for LPS core of mesophilic Aeromonas strains. The lanes contained the same strains as the lanes in panel A. (C) LPS from A. hydrophila AH-3 (wild type) (lane 1), E. coli K-12 strain CLM4 (lane 2), strain CLM4 with the COS-O34 plasmid (lane 3), and strain CLM4 with the COS-O34 and pGEMT-Gne plasmids (lane 4). All the strains were grown at 20°C, and strains with pBAD33 plasmids were grown under induced conditions.