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(a) AFM height image (2.5 by 2.5 μm) of a P. aeruginosa PAO1 cell obtained in contact mode by using a V-shaped AFM cantilever with a pyramid-shaped tip by increasing the loading force F0 in a step-wise fashion. The cross section corresponds to the black line shown in the AFM image along the length of the cell. (b) AFM deflection image of the same cell collected after the image shown in panel a, using a small F0 value of 1 nN.
