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Denaturing gel electrophoresis (SDS-PAGE) of concentrated culture supernatants: CipA fragments containing cohesins. Fifteen micrograms of protein was applied to the gel: SM1, 5.02 μl; SM4, 10.7 μl; SM5, 8.1 μl; SM6, 19.2 μl; and wild type (WT), 11.35 μl. Proteins containing cohesin modules were detected with His tag-Cel9N and anti-His antibodies. The numbers indicate molecular masses (kDa) of the protein mass standard (lane M).
