TABLE 3.
Activities of transcriptional fusions of rctA and virB promoters in different genetic backgrounds
| Strain (relevant genotype)a | Sp act (nmol min−1 mg of protein−1)b from the indicated promoter in β-glucuronidase fusion with:
|
|||||
|---|---|---|---|---|---|---|
| WT virB
|
virB- RBM
|
virB-10m
|
||||
| PvirB | PrctA | PvirB | PrctA | PvirB | PrctA | |
| CFN2001 Tn5.C (rctA+ rctB+) | 59 ± 9 | 240 ± 18 | 220 ± 72 | 39 ± 13 | ND | 215 ± 9 |
| CFN2001 Tn5.2 (rctA rctB+) | 257 ± 18 | 48 ± 15 | 237 ± 109 | 32 ± 22 | ND | 221 ± 16 |
| CFN2001 Tn5.6 (rctA+ rctB++) | 283 ± 58 | 67 ± 24 | 188 ± 56 | 29 ± 11 | ND | 229 ± 16 |
| CFN2001 Tn5.2/pSSH05 [rctA+(k) rctB+] | 87 ± 14 | 323 ± 55 | NA | NA | NA | NA |
| CFN2001 (rctA rctB) | 225 ± 57 | 33 ± 7 | 213 ± 68 | 55 ± 30 | ND | 220 ± 7 |
| CFN2001/pTErctA [rctA+(tr) rctB] | 2 ± 2 | 405 ± 100 | 247 ± 104 | 55 ± 30 | ND | 208 ± 24 |
| CFN2001/pTErctB [rctA rctB+(tr)] | 471 ± 51 | 4 ± 3 | 232 ± 77 | 24 ± 7 | ND | 230 ± 20 |
a
+, wild-type expression; ++, unregulated expression; (tr), expression from the trp promoter; (k) expression from the pET16B promoter.
b
Specific β-glucuronidase activities are expressed as means ± standard deviations of results from at least three independent experiments. WT virB, wild-type virB (on fragment pVT); virB-RBM, virB with a mutated RBM box (on fragment pVT-RBM); virB-10m, virB with a mutated −10 box (on fragment pVT-10m); ND, not determined; NA, not applicable.