TABLE 3.
Oligonucleotides used in this study
| Oligonucleotide | 5′ to 3′ sequencea | Purpose |
|---|---|---|
| AttTn7Ext | TGTTGGTTCACATCC | Tn7 insert amplification |
| ErmAnch1 | TACTTATGAGCAAGTATTGTC | Tn7 insert amplification |
| AMtoZfornew | GACAAAATGACTCTAGATTTCTAATTATTATCCTAATCTGC | Mutagenesis |
| AMtoZrevnew | GCAGATTAGGATAATAATTAGAAATCTAGAGTCATTTTGTC | Mutagenesis |
| BMtoZfor | TGGATTTGATTTTTTTCTAAGAGTTTTCATTGGTAATG | Mutagenesis |
| BMtoZrev | TACCAATGAAAACTCTTAGAAAAAAATCAAATCCATCG | Mutagenesis |
| TnMtoZfor | TTTATGGATGTATTCGCTCTACCATTTAGTTTACGCCGC | Mutagenesis |
| TnMtoZrev | GCGTAAACTAAATGGTAGAGCGAATACATCCATAAAAGC | Mutagenesis |
| pKmGFPEcoFor | GAATTCGTTGTGTCTCAAAATCTCTG | gfp amplification |
| pKmGFPEcoRev | GAATTCGGATATAGTTCCTCCTTTCAGC | gfp amplification |
a
Underlined regions indicate ATG to TAG null mutations.