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Tobramycin accumulates in the PA1874-1877 deletion mutant biofilm but not the wild-type biofilm. Wild-type (WT) and mutant (Δ1874-1877) biofilms were exposed to 200 μg/ml tobramycin. The control biofilms were not exposed to tobramycin. The amount of tobramycin that had accumulated in the biofilms was observed as a zone of clearing on the lawn of E. coli, using the white disks as focal points.
