FIG. 3.

Analysis of PA1874 gene expression by RT-PCR. Expression of the PA1874 gene in planktonic (lanes 6 and 7) and biofilm (lanes 8 and 9) cells of P. aeruginosa PA14 measured by RT-PCR amplification of RNA with primers specific to PA1874 gene (lanes 6 to 9) and rpoD gene (control) (lanes 2 to 5). The RNA templates were isolated from planktonic and biofilm cells, and the different amounts of the templates were used (undiluted in lanes 2, 4, 6, and 8; 1:10 dilution in lanes 3, 5, 7, and 9; data with 1:100 dilution not shown). DNA size markers (0.1 to 12 kb) (1 kb plus DNA ladder; Invitrogen) are shown in lane 1.