TABLE 3.
Introduction of divIB, divIC, and ftsL at the ami locus in strains R6 and B2
| Parental strain | Genotype | Growtha in:
|
Average chain length (μm)b in:
|
||
|---|---|---|---|---|---|
| CD medium | TH medium | CD medium | TH medium | ||
| R6 | Wild type | + | + | 1.5 | 1.6 |
| ami::PM-divIB | + | + | 1.6 | 1.9 | |
| ami::PM-divIC | + | + | 1.4 | 1.9 | |
| ami::PM-ftsL | + | + | 1.4 | 3.3 | |
| B2 | divIB::cat | − | + | − | 6.6 |
| divIB::cat ami::PfcsK-divIB | + | + | 1.4 | 1.8 | |
| divIB::cat ami::PfcsK-divIC | − | + | − | 4.2 | |
| divIB::cat ami::PfcsK-ftsL | + | + | 3.4 | 6.5 | |
a
Growth was monitored at 590 nm in 12-well plates. +, doubling time of about 45 min; −, absence of growth for 35 h.
b
Strains were cultivated to an optical density at 600 nm of between 0.3 and 0.5, and 200 particles were measured for each strain.