FIG. 4.

Proteolytic digestion of WT CRP and CRP* variants by subtilisin. Each CRP (1.12 mg/ml) was digested with various amounts of subtilisin; the black wedge indicates twofold serial dilution of subtilisin, and the first lane contained 11.2 μg of subtilisin/ml (CRP/subtilisin, 1:100). An asterisk indicates the control lane showing each CRP treated identically but without subtilisin. Arrows indicate the uncut CRP band. The concentration of cAMP used for WT CRP was 100 μM. The reaction was carried out at 25°C for 24 h.