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Nonpolar distribution of IcsAi532 and IcsAi563 mutants on the surface of S. flexneri. IF microscopy of IcsA surface distribution. Log-phase cultures of S. flexneri strains expressing either IcsAWT or IcsAi were formalin fixed and labeled with anti-IcsA antibodies and Alexa 488-conjugated goat antirabbit secondary antibodies. For clarity, the IF image for each strain is accompanied by an overlay of the IF image with the corresponding phase-contrast image. Bar = 10 μm.
