TABLE 3.
F-actin comet tail formation and N-WASP recruitment by R-LPS S. flexneri ΔicsA ΔrmlD strains expressing IcsAi mutants
| Protein expressed | Phenotype of S. flexneri ΔicsA ΔrmlD straina
|
|
|---|---|---|
| F-actin tail formation | N-WASP recruitment | |
| IcsAWT | +++ | +++ |
| IcsAi185 | ++ | ++ |
| IcsAi219 | − | − |
| IcsAi226 | ++ | ++ |
| IcsAi228 | ++ | ++ |
| IcsAi244 | − | − |
| IcsAi248 | − | − |
| IcsAi268 | − | − |
| IcsAi271 | − | + |
| IcsAi297 | − | − |
| IcsAi330a | − | − |
| IcsAi330b | − | − |
| IcsAi342 | − | − |
| IcsAi346 | − | +/− |
| IcsAi381 | − | − |
| IcsAi532 | ++ | ++ |
| IcsAi563 | ++ | ++ |
| IcsAΔ103-320 | − | + |
| IcsAΔ508-730 | +/− | + |
| IcsAΔ103-507 | − | + |
a
+++, WT N-WASP recruitment/F-actin comet tail formation; ++, 50 to 90% reduction in N-WASP recruitment/F-actin tail formation relative to IcsAWT; +, >90% reduction in N-WASP recruitment/F-actin tail formation; +/−, F-actin capping; −, N-WASP/F-actin tail formation not detected. Two independent experiments (n = 20) were carried out.