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Antagonistic effects of 3OC12-HSL and rosiglitazone on mRNA levels for proinflammatory genes. A549 cells were incubated in the absence or presence of 25 μm 3OC12-HSL, either alone or in the presence of various concentrations of rosiglitazone (Rosi) for 6 h. RNA was extracted and converted into cDNA, and mRNA levels for a panel of proinflammatory genes were assessed by RT-PCR. The mRNA encoding the ribosomal protein L19 was analyzed as a loading control.
