TABLE 1.
Gene product | Sequence (5′ to 3′) of:
|
|
---|---|---|
Forward primer | Reverse primer | |
Murine gene products | ||
GAPDH | AAGGTCGGAGTCAACGGATT | TTGATGACAAGCTTCCCGTT |
COX-2 | CCCCCACAGTCAAAGACACT | GAGTCCATGTTCCAGGAGGA |
KC | GCTGGGATTCACCTCAAGAA | TGGGGACACCTTTTAGCATC |
IL-6 | ACTTCACAAGTCCGGAGAGG | GTTGAAGATATGAATTAGAG |
IL-1α | CGTCAGGCAGAAGTTTGTCA | GTGCACCCGACTTTGTTCTT |
PPARα | TGCAAACTTGGACTTGAACG | AATCCCCTCCTGCAACTTCT |
PPARβ/δ | GCAGCCTCTTCCTCAATGAC | GTACTGGCTGTCAGGGTGGT |
PPARγ | CTGGCCTCCCTGATGAATAA | ACGTGCTCTGTGACGATCTG |
RXRα | CTCCTATCAGCACCCTGAGC | TGTTGTCTCGGCAGGTGTAG |
RXRβ | CTCCTCATTGCGTCCTTCTC | CCTGCTGCTCAGGGTACTTC |
RXRγ | AATGCTCTTGGCTCTCCGTA | TGAAGAAGCCTTTGCAACCT |
FXR | GGCAGAATCTGGATTTGGAA | GTGAGCGCGTTGTAGTGGTA |
PXR | TTTCAGAAGGGCCATGAAAC | CCTGCAGAAACTCTGGAAGC |
CAR | GGAGGACCAGATCTCCCTTC | GTTCAGAATCAGCGCCATTT |
Human gene products | ||
L19 | GCTGATCAAGGATGGGCTGA | CGGGAATGGACAGTCACAGG |
COX-2 | TGAAACCCACTCCAAACACA | AACTGATGCGTGAAGTGCTG |
IL-8 | TCTGCAGCTCTGTGTGAAGG | ATTGCATCTGGCAACCCTAC |
IL-6 | AAAGAGGCACTGGCAGAAAA | AAAGCTGCGCAGAATGAGAT |
IL-1α | GTAAGCTATGGCCCACTCCA | AGCAGCCGTGAGGTACTGAT |
PPARα | CTGGAAGCTTTGGCTTTACG | GTTGTGTGACATCCCGACAG |
PPARβ/δ | GTGTGGAAGCAGTTGGTGAA | GCGTTGAACTTGACAGCAAA |
PPARγ | TTCAGAAATGCCTTGCAGTG | CACCTCTTTGCTCTGCTCCT |
RXRα | TTCTCCACCCAGGTGAACTC | CTTGGTGAAGGAAGCCATGT |
RXRβ | CCTTCTCACACCGATCCATT | CCTGCTGCTCAGGGTACTTC |
RXRγ | TGTGGTCAACAGTGTCAGCA | CCTCACTCTCAGCTCGCTCT |
FXR | TTGCTTTGCTGAAAGGGTCT | GAGGATTTTCAGGCTGGTGA |
PXR | CTGATGGACGCTCAGATGAA | AGGGAGATCTGGTCCTCGAT |
CAR | GCAGCTGTGGAAATCTGTCA | AGGCCTAGCAACTTCGCATA |