TABLE 1.
Strains and plasmidsa
| Strain or plasmidb | Descriptionc | Source |
|---|---|---|
| Strains | ||
| S. enterica | ||
| TR6583 | metE205 ara-9 | J. Roth |
| DM10310 | ara-9 (wild type) | |
| DM9678 | apbE42::Tn10d(tet) | |
| DM9682 | rseC::kan | |
| DM9684 | cyaY::cat | |
| DM10780 | ara-9 apbC55::Tn10d(tet) rseC::kan | |
| DM10779 | ara-9 apbC::MudJ (kan) apbE42::Tn10d(tet) | |
| DM10296 | ara-9 yggX::Gm | |
| DM10300 | ara-9 apbC55::Tn10d(tet) | |
| DM10302 | ara-9 apbC55:Tn10d(tet) yggX::Gm | |
| DM10696 | ara-9 iscR2::MudJ (kan) | |
| DM10697 | ara-9 apbC55:Tn10d(tet) iscR2::MudJ (kan) | |
| DM10325 | ara-9 ΔiscRSUA::cat | |
| DM10681 | ara-9 apbC55::Tn10d(tet) Δisc5::cat | |
| DM10326 | ara-9 sufS::cat | |
| DM10682 | ara-9 apbC55::Tn10d(tet) sufS::cat | |
| DM10667 | ara-9 iscA1::MudJ (kan) | |
| DM10683 | ara-9 apbC55::Tn10d(tet) iscA1::MudJ (kan) | |
| DM10604 | ara-9 apbC::MudJ (kan) | |
| DM10608 | ara-9 apbC::MudJ (kan) iscR11 | |
| JE10432 | ara-9 iscR::kan | J. C. Escalante-Semerena |
| DM10698 | ara-9 ΔiscR11 | |
| JE10435 | ara-9 apbC55::Tn10d(tet) ΔiscR11 | J. C. Escalante-Semerena |
| DM10685 | ara-9 apbC55::Tn10d(tet) yggX::Gm iscR11 | |
| DM10474 | ara-9 apbC55::Tn10d(tet) stm2545::Tn10d(cat) iscR6 | |
| DM10476 | ara-9 apbC55::Tn10d(tet) stm2545::Tn10d(cat) iscR7 | |
| DM10480 | ara-9 apbC55::Tn10d(tet) stm2545::Tn10d(cat) iscR8 | |
| DM10482 | ara-9 apbC55::Tn10d(tet) stm2545::Tn10d(cat) iscR9 | |
| DM10484 | ara-9 apbC55::Tn10d(tet) stm2545::Tn10d(cat) iscR10 | |
| DM10771 | ara-9 staC::Tn10d(tet) stm2545::Tn10d(cat) yggX::Gm | |
| DM10772 | ara-9 apbC::MudJ (kan) staC::Tn10d(tet) stm2545::Tn10d(cat) yggX::Gm | |
| DM10770 | ara-9 apbC::MudJ (kan) stm2545::Tn10d(Cm) yggX::Gm iscR7 | |
| DM10769 | ara-9 staC::Tn10d(tet+) stm2545::Tn10d(cat+) yggX::Gm iscR7 | |
| E. coli | ||
| C43(λDE3) | F−ompT gal hsdSB(rB− mB−) dcm lon | J. C. Escalante-Semerena |
| JE10465 | F−ompT gal hsdSB(rB− mB−) dcm lon apbC::kan+ | J. C. Escalante-Semerena |
| DH5α/F′ | F′/endA1 hsdR17(rK− mK+) supE44 thi-1 recA1 gyrA (Nar1r) relA1 Δ(lacZYA-argF)U169 deoR[80dlac Δ(lacZ)M15] | New England Biolabs |
| Plasmids (vectors) | ||
| pSU19 (pSU19) | None | 2 |
| pIscR1 (pSU19) | iscR (S. enterica) | 52 |
| pIscS1 (pSU19) | iscS (S. enterica) | 52 |
| pIscA1 (pSU19) | iscA (S. enterica) | 52 |
| pIscA-orf3 (pSU19) | iscA-hscB-hscA-fdx-orf3 (S. enterica) | 52 |
| pIscU1 (pSU19) | iscU (S. enterica) | |
| pTCU21 (pBAD30) | tcuABC (S. enterica) | 31 |
| pCTH-ApbC (pET20b) | apbC (S. enterica) | 51 |
| pDB1282 (pAra13) | iscSUA-hscB-hscA-fdx-orf3 (A. vinelandii) | Dennis Dean |
| pHscB-orf3 (pSU19) | hscB-hscA-fdx-orf3 (S. enterica) | |
| pGSO164 (pBAD) | sufABCDSE (E. coli) | 43 |
| pIsc2 (pFZY1) | iscR operator | 26d |
| pTCU55 (pET15b) | tcuB | 30 |
| pApbC (pSU19) | apbC |
a
Unless indicated otherwise in the description, all strains are S. enterica serovar Typhimurium LT2 constructed for this study.
b
All S. enterica strains constructed for this study are derivatives of TR6583 that were transduced to metE+. The TR6583 strain was from the Escalante-Semerena laboratory.
c
Genotypes are given for strains, and inserts (hosts) are given for plasmids. The following alleles have been previously described: apbC55::Tn10d (46), cyaY::Cm (57), rseC::Kan (3, 53), apbE42::Tn10d(Tc) (4), yggX::Gm (19), iscR2::MudJ (52), Δisc5::Cm (53), and iscA2::MudJ (52). Tc, tetracycline; Cm, chloramphenicol; Gm, gentamicin; Kan, kanamycin.
d
Source also includes Lewis et al. (submitted).