TABLE 2.
An apbC mutant is unable to grow on tricarballylate medium
| Strain | Relevant genotypeb | Doubling time (h) on medium containinga:
|
Ratio of doubling timesd | |
|---|---|---|---|---|
| Tricarballylatec | Glucosec | |||
| DM10310 | Wild type | 1.7 ± 0.1 | 2.1 ± 0.1 | 0.81 |
| DM10667 | iscA-hscAB-fdx-orf3 | 1.9 ± 0.1 | 2.1 ± 0.1 | 0.90 |
| DM10325 | iscSUA-hscAB-fdx-orf3 | 4.5 ± 0.2 | 4.3 ± 0.1 | 1.1 |
| DM10326 | sufS | 1.8 ± 0.1 | 2.2 ± 0.0 | 0.82 |
| DM10300 | apbC | NG | 2.1 ± 0.0 | |
a
Doubling times were calculated using the formula μ = ln(X/Xo)/T, where μ is the growth rate, X and Xo are optical density measurements at 650 nm, T is the time between the absorbance readings X and Xo, and the doubling time (g) was (ln 2)/μ (39). Values are averages of three independent cultures. NG, no growth.
b
Relevant genotypes indicate the genes that are defective due to the presence of a polar mutation or deletion.
c
The defined medium included the indicated carbon source and was supplemented with thiamine and nicotinic acid.
d
Ratio of the doubling time on tricarballylate to the doubling time on glucose.