TABLE 3.
Inactivation of IscR derepresses the isc operona
| Strain | Relevant genotype | β-Galactosidase activity (Miller units) |
|---|---|---|
| DM10310 | Wild type | 70 ± 4 |
| DM10300 | apbC | 70 ± 2 |
| JE10432 | iscR::Kan | 390 ± 72 |
| DM10474 | apbC iscR6 | 270 ± 28 |
| DM10476 | apbC iscR7 | 430 ± 47 |
| DM10480 | apbC iscR8 | 350 ± 31 |
| DM10482 | apbC iscR9 | 340 ± 28 |
| DM10484 | apbC iscR10 | 290 ± 22 |
All strains contained a plasmid that carried the isc reporter-lacZ fusion as described in the text. Strains were grown in LB-ampicillin medium to the mid-exponential phase of cell growth. β-Galactosidase assays were performed as described in Materials and Methods.