TABLE 1.
Plasmids and strains used in this study
| Strain or plasmid | Genotype, characteristics, or construction | Source or reference |
|---|---|---|
| E. coli strains | ||
| TAM1 | mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Active Motif |
| DH5α | endA1 hsdR17(rK− mK+) glnV44 thi-1 recA1 gyrA (Nalr) relA Δ(lacIZYA-argF)U169 deoR [φ80dlacΔ(lacZ)M15] | 48 |
| TOP10 F′ | F′ [lacIq Tn10 (Tetr)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| V. fischeri strains | ||
| ES114 | Wild type | 4 |
| KV2566 | ES114 att Tn7::PsypA::lacZ | 15a |
| Plasmids | ||
| pJET1 | Commercial cloning vector, Ampr | Fermentas |
| pCR2.1-TOPO | Commercial cloning vector, Ampr Kanr | Invitrogen |
| pKV69 | Mobilizable vector, Cmr Tetr | 47 |
| pKG63 | pKV69 containing rscS1 under control of PlacZ, Cmr Tetr | 15a |
| pKG92 | pKV69 containing rscS1(ΔPP-large), Cmr Tetr | This study |
| pKG94 | pKV69 containing rscS1(ΔPP-small), Cmr Tetr | This study |
| pKG99 | pKV69 containing rscS1(H323A), Cmr Tetr | This study |
| pKG100 | pKV69 containing rscS1(F353A), Cmr Tetr | This study |
| pKG102 | pKV69 containing rscS1(N345A), Cmr Tetr | This study |
| pKG107 | pKV69 containing rscS1(H412Q), Cmr Tetr | This study |
| pKG108 | pKV69 containing rscS1(H867Q), Cmr Tetr | This study |
| pKG115 | pKV69 containing rscS1(ΔN-term), Cmr Tetr | This study |
| pKG116 | pKV69 containing rscS1(L330R), Cmr Tetr | This study |
| pKG123 | pKV69 containing rscS1(S9T), Cmr Tetr | This study |
| pKG124 | pKV69 containing rscS1(S9A), Cmr Tetr | This study |
| pKG125 | pKV69 containing rscS1(I13A), Cmr Tetr | This study |
| pKG127 | pKV69 containing rscS1(D709A), Cmr Tetr | This study |