TABLE 3.
Heat resistance of B. subtilis strainsa
| Strain | Enzyme deficiency | Cell titer (CFU/ml)b | Spore titer (CFU/ml)c | Sporulation efficiency (%)d |
|---|---|---|---|---|
| 1A1 | None (parental strain) | 1.3 × 108 | 5.5 × 107 | 42 |
| LMM24 | TrxA | 6.2 × 107 | <1 × 102 | <0.0002 |
| LMM241 | TrxA | 6.7 × 107 | 2 × 102 | <0.0003 |
| LMM82 | TrxA BdbD | 1.2 × 106 | 2.8 × 105 | 23 |
Data indicate the heat resistance of B. subtilis strains grown for sporulation in NSMP supplemented with deoxyribonucleosides, cysteine, and methionine.
Cell titer values are CFU/ml before treatment.
Spore titers are CFU/ml after heat treatment.
Sporulation efficiency was calculated as the spore titer divided by the cell titer.