TABLE 2.

Real-time PCR for expression of T3SS-related regulatory genes: hilA, hilC, hilD, and hilE gene expression in the wild-type strain in the presence or absence of 100 mM NaCl

Presence of NaCl	Expression levels of genesa
	hilA	hilC	hilD	hilE
−	1.00 ± 0.10	1.00 ± 0.09	1.00 ± 0.07	1.00 ± 0.03
+	5.70 ± 0.67	3.00 ± 0.43	4.69 ± 0.54	1.16 ± 0.10

^amRNA levels of the genes from cultures grown in TY medium supplemented with 100 mM NaCl or with no added salt are indicated. The values for the relative levels obtained under the two conditions were determined by defining the level in TY medium with no added salt as 1.