TABLE 3.
Real-time PCR for expression of T3SS-related regulatory genes: hilA, hilC, and hilD gene expression in hilC, hilD, and hilC hilD deletion mutants
| Strain | Presence of NaCl | Expression levels of genesa
|
||
|---|---|---|---|---|
| hilA | hilC | hilD | ||
| Wild type | − | 1.00 ± 0.08 | 1.00 ± 0.16 | 1.00 ± 0.03 |
| + | 5.27 ± 0.37 | 5.97 ± 1.09 | 3.56 ± 0.13 | |
| ΔhilC | − | 0.14 ± 0.04 | 0.00 ± 0.00 | 0.24 ± 0.02 |
| + | 1.40 ± 0.28 | 0.00 ± 0.00 | 1.36 ± 0.10 | |
| ΔhilD | − | 0.00 ± 0.00 | 0.03 ± 0.01 | 0.00 ± 0.00 |
| + | 0.00 ± 0.00 | 0.06 ± 0.01 | 0.00 ± 0.00 | |
| ΔhilC ΔhilD | − | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| + | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | |
a
See Table 2, footnote a.