TABLE 5.

Real-time PCR for expression of T3SS-related regulatory genes: hilA and hilD gene expression in mutants deficient for barA, sirA, or envZ

Strain	Presence of NaCl	Expression levels of genesa
		hilA	hilD
Wild type	−	1.00 ± 0.24	1.00 ± 0.04
	+	3.74 ± 1.24	2.72 ± 0.12
ΔbarA	−	0.01 ± 0.00	0.06 ± 0.01
	+	0.16 ± 0.05	0.32 ± 0.01
ΔsirA	−	0.01 ± 0.00	0.06 ± 0.00
	+	0.27 ± 0.06	0.27 ± 0.02
ΔenvZ	−	0.42 ± 0.07	0.46 ± 0.02
	+	4.80 ± 0.88	2.32 ± 0.16

^aSee Table 2, footnote a.