TABLE 6.

Real-time PCR for expression of T3SS-related regulatory genes: hilA, csrA, csrB, and csrC gene expression in mutants deficient for barA, sirA, or envZ

Strain	Presence of NaCl	Expression levels of genesa
		hilA	csrA	csrB	csrC
Wild type	−	1.00 ± 0.22	1.00 ± 0.14	1.00 ± 0.05	1.00 ± 0.59
	+	8.36 ± 1.61	1.11 ± 0.12	1.13 ± 0.05	0.52 ± 0.28
ΔbarA	−	0.02 ± 0.00	0.95 ± 0.11	0.14 ± 0.01	0.19 ± 0.09
	+	0.29 ± 0.06	0.64 ± 0.07	0.30 ± 0.01	0.16 ± 0.10
ΔsirA	−	0.02 ± 0.01	0.94 ± 0.11	0.13 ± 0.01	0.18 ± 0.08
	+	0.13 ± 0.02	0.74 ± 0.08	0.17 ± 0.04	0.13 ± 0.05
ΔenvZ	−	0.83 ± 0.20	1.14 ± 0.12	0.90 ± 0.09	0.63 ± 0.32
	+	8.87 ± 1.59	0.87 ± 0.10	1.31 ± 0.06	0.41 ± 0.17

^aSee Table 2, footnote a.