TABLE 1.
Double mutations in lux box critical bases that conserve the palindrome cause severe loss of promoter function
| lux box nucleotide substitution(s) | Percent (±SD) of wild-type activity |
|---|---|
| Critical-base substitutionsa | |
| C3A | 70 (12) |
| G18T | 54 (9) |
| C3A, G18T | 2.4 (0.5) |
| C3G | 82 (5) |
| G18C | 14 (2) |
| C3G, G18C | 0.1 (0.4) |
| C3T | 58 (6) |
| G18A | 60 (3) |
| C3T, G18A | 21 (1) |
| T4A | 39 (5) |
| A17T | 42 (2) |
| T4A, A17T | <0.1 |
| T4C | 79 (7) |
| A17G | 84 (5) |
| T4C, A17G | 21 (4) |
| T4G | 28 (3) |
| A17C | 36 (5) |
| T4G, A17C | <0.1 |
| G5C | 9 (1) |
| C16G | 43 (8) |
| G5C, C16G | 0.3 (0.3) |
| Noncritical-base substitutions | |
| A7T | 84 (5) |
| T14A | 99 (15) |
| A7T, T14A | 67 (12) |
| A10C | 94 (18) |
| T11G | 96 (2) |
| A10C, T11G | 77 (4) |
| A10T | 108 (6) |
| T11A | 107 (8) |
| A10T, T11A | 91 (0.4) |
a
The critical bases are positions 3 to 5 and 16 to 18 of the lux box.