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(A) Domain organization of EspA protein depicted by SMART analysis (26, 46). PAS/PAC, energy-sensing domains; HisKA histidine kinase dimerization domain; HATPase_c, histidine kinase ATPase domain; REC, receiver domain. (B) Constructs for analysis of EspA kinase activity in vitro. (C) Mutations generated in the espA locus for in vivo analyses.
