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. 2008 Apr 4;190(13):4416–4426. doi: 10.1128/JB.00265-08

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FIG. 2. — Analysis of EspA kinase activity. (A) Autoradiograph of GST-EspA_kin (lane 1), GST-EspA_kin(H407A) (lane 2), GST-EspA_kin-rec (lane 3), and GST-EspA_kin-rec(D696A) (lane 4) incubated in the presence of [γ-³²P]ATP. (B) Coomassie-stained gel corresponding to panel A. (C) TLC analysis of [α-³²P]ADP produced by [α-³²P]ATP (lane 1), GST-EspA_kin (lane 2), GST-EspA_kin-rec (lane 3), and GST-EspA_kin-rec(D696A) (lane 4). (D) Quantification of [α-³²P]ADP signal in panel C as an average from triplicate experiments.