Figure 3.

Confirmation of SAGE results by Northern blot analysis. Representative Northern blots are shown with RNA prepared from cells induced (“+”) for 9 hr or uninduced (“−”). (A) RNA from two independent p53-inducible clones was analyzed to confirm induction of transcripts by p53. No induction of these transcripts was observed in RNA prepared from cells with inducible mutant p53 genes (data not shown). (B) RNA from two independent p73-inducible clones was used to assay induction of transcripts by p73.