Table 2.
Classification of p53-regulated genes
| Gene | Class | Time | Adr. Ind. | 5-FU Ind. | p53-dep. | p73-Ind. |
|---|---|---|---|---|---|---|
| Cav | I | E | 1 | 1 | − | − |
| DR5 | I | E | 1 | 2 | − | − |
| Gpc | I | E | 1 | 1 | + | − |
| 14-3-3σ* | I | E | 2 | 2 | − | + |
| p21 | I | E | 2 | 2 | + | + |
| HO1 | I | I | 2 | 1 | − | − |
| Htk | I | I | 1 | 1 | − | − |
| Bax* | I | I | 2 | 2 | − | − |
| PIG3* | I | I | 2 | 2 | + | − |
| Jun-B | II | E | 0 | 0 | − | + |
| Mdm2* | II | E | 2 | 2 | + | − |
| PET-3 | II | E | 2 | ND | − | − |
| PET-7 | II | E | 2 | ND | − | − |
| ATDC | II | E | 0 | ND | N/A | + |
| B61 | II | I | 2 | 2 | − | − |
| PET-1 | II | I | 1 | 0 | − | − |
| PET-8 | II | I | 1 | 1 | − | − |
| PET-4 | II | I | 1 | 2 | − | − |
| Gadd45* | II | L | 1 | 0 | − | + |
| RTP | II | I | 1 | 1 | − | + |
| IGBP3* | II | I | 1 | 0 | N/A | − |
| ET2 | II | I | 0 | 0 | − | − |
| Mat8 | II | I | 2 | 0 | + | − |
| PET-9 | II | I | 1 | ND | − | − |
| Fas* | II | N/A | 1 | 0 | − | − |
| PET-11 | II | ND | 1 | ND | − | − |
| Cln G1* | III | N/A | 0 | 0 | N/A | − |
| Tsp | III | E | 1 | 1 | − | − |
| PET-2 | III | I | 0 | ND | N/A | − |
| PET-5 | III | I | 0 | ND | N/A | − |
| PET-6 | III | I | 0 | ND | N/A | − |
| IPK | III | I | 1 | ND | − | − |
| CatD* | III | N/A | 0 | 0 | N/A | ND |
| PET-13 | III | ND | 1 | ND | − | ND |
| Tap1 | ND | I | ND | ND | ND | − |
| EVPL | ND | I | ND | ND | ND | − |
Class: class I, induced by Ad-p53 wt in all five colorectal cancer cell lines studied; class II, induced in only a subset of these lines; class III, induced in none of the lines; N/A, not applicable; ND, not determined. Time: E, early (detectably induced within 3 hr of doxycycline withdrawal); I, intermediate (detectably induced after 6 hr); L, late (detectably induced only after 12 hr). Adriamycin (Adr.) or 5-FU induced (ind.): 2, induced in two wt p53 cell lines; 1, induced in one cell line; 0, induced in neither cell line. p53 dependence (dep.) was determined only for transcripts induced by adriamycin or 5-FU in HCT116 cells. “+” denotes that induction was reduced substantially or absent in p53 KO cells, and “−” indicates that comparable induction was observed in the p53 KO cells. p73 induced; “+” denotes that transcript was induced in two p73 wt clones, and “−” indicates that induction was not observed in both clones.
Genes not identified herein but shown to be p53-regulated in other studies. All other genes were identified through the SAGE analysis reported in this paper.