FIG. 1.

Western blot analysis of wild-type S. uberis grown in the presence and absence of the Lsp inhibitor globomycin. Globomycin (100 μg/ml) was added where appropriate to bacterial cultures at the start of exponential phase. Whole-cell lysates were prepared from bacteria cultured in the presence (lanes 3) and absence (lanes 2) of globomycin grown to the start of stationary phase (A) or for 24 h (B). Markers are shown with molecular masses (lanes 1). Arrows 1 and 2, higher- and lower-molecular-mass forms of MtuA, respectively, recognized by specific antiserum.