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Deletions of virB genes are nonpolar on transcription of downstream genes. PCR amplicons from RNA isolated from wild-type B. abortus and its isogenic mutants with primers for virB1, virB6, virB11, and gyrA are shown. The genotype of each mutant is listed at the top of the figure (Δ1, ΔvirB1; Δ2, ΔvirB2, etc.; WT, wild type). For each strain, a PCR was performed with reverse transcriptase (a) or without reverse transcriptase (b); lane c, wild-type genomic DNA control.
