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Western blot of wild type and deletion mutant strains. All strains were grown under expression conditions described by Patey et al. (34). Expression was detected with polyclonal antisera raised against VirB5 (α-VirB5), VirB8 (α-VirB8), VirB9 (α-VirB9), VirB10 (α-VirB10), VirB11 (α-VirB11), VirB12 (α-VirB12), and Bcsp31 (α-Bcsp31) (loading control).
