TABLE 2.
Primers used in this worka
| Primer name | Sequence (restriction enzyme) | Fragment |
|---|---|---|
| VirB3up-F | GTTGTCGTGCCCATGATC | virB3 upstream |
| VirB3up-R | CCCGGGGATCTGTGTCCCTTTACC (SmaI) | virB3 upstream |
| VirB3dn-F | CCCGGGATGGGCGCTCAATCCAAA (SmaI) | virB3 downstream |
| VirB3dn-R | CTGCAGTAATGATATTCACCCATG (PstI) | virB3 downstream |
| VirB4up-F | ATCTGCGATCTGGAGCAT | virB4 upstream |
| VirB4up-R2 | CCCGGGTTATGATTCTCTTTTGCGC (SmaI) | virB4 upstream |
| VirB4dn-F | CCCGGGCACTATGAAGAAGATAAT (SmaI) | virB4 downstream |
| VirB4dn-R | CTGCAGCATATTCATGGTGTTGGA (PstI) | virB4 downstream |
| VirB5up-F3 | TCTAGAGGGACTATTCTTGCCATT (XbaI) | virB5 upstream |
| VirB5up-R2 | CTGCAGAGTGTCACCTTCCTGTTG (PstI) | virB5 upstream |
| VirB5dn-F2 | CTGCAGCTACCCGACTAAGGAGTA (PstI) | virB5 downstream |
| VirB5dn-R2 | GTCGACTGCCACCGTATTCATAGA (SalI) | virB5 downstream |
| VirB6up-F2 | TCTAGAATTGCCGAAGTCGGGGAC (XbaI) | virB6 upstream |
| VirB6up-R2 | CTGCAGCACGAAACTCCTTCACCC (PstI) | virB6 upstream |
| VirB6dn-F2 | CTGCAGGGCCTTACACGCGGCGGC (PstI) | virB6 downstream |
| VirB6dn-R3 | GTCGACGTGACATTGAAACCCAGCG (SalI) | virB6 downstream |
| VirB7up-F2 | TCTAGAAAATTCATGCTGATTGCT (XbaI) | virB7 upstream |
| VirB7up-R2 | CTGCAGTATGATTACCTGCGGTGC (PstI) | virB7 upstream |
| VirB7dn-F2 | CTGCAGTCATGTTTGGACGCAAAC (PstI) | virB7 downstream |
| VirB7dn-R2 | GTCGACGTGGGAGAGCGTCATGGA (SalI) | virB7 downstream |
| VirB8up-F | CGCAGCCATGCTCTTTGA | virB8 upstream |
| VirB8up-R | CCCGGGGATTAGTCCTCGTAAGTG (SmaI) | virB8 upstream |
| VirB8dn-F | CCCGGGGCAATGAAAAGATTCCTG (SmaI) | virB8 downstream |
| VirB8dn-R | CTGCAGCGATTTGATGACGCGCTT (PstI) | virB8 downstream |
| VirB9up-F2 | TCTAGACTGGCACATCATGGGCGA (XbaI) | virB9 upstream |
| VirB9up-R2 | CTGCAGTGCACCACTCCCATTTCT (PstI) | virB9 upstream |
| VirB9dn-F2 | CTGCAGATGACACAGGAAAACATT (PstI) | virB9 downstream |
| VirB9dn-R2 | GTCGACAGGTTGATCTGATTTGAA (SalI) | virB9 downstream |
| VirB10up-F | GCGGCCGCAACAACGCACTGGATCGC (NotI) | virB10 upstream |
| VirB10up-R | GGATCCTGCAGGTTCTCCCCGGGC (BamHI) | virB10 upstream |
| VirB10dn-F | GATATCATGATGTCAAACCGAAGT (EcoRV) | virB10 downstream |
| VirB10dn-R | GTCGACTATGGCGGCCAGTGCCGT (SalI) | virB10 downstream |
| virB8351F | GTCGACGCCCACTCTTGCCTATG (SalI) | virB11 upstream |
| virB9822R | CTGCAGTCATTCACTTCGGTTTGACATCATACAC (PstI) | virB11 upstream |
| virB10850 | CTGCAGAACGGGCACTGTCACTGC (PstI) | virB11 downstream |
| virB12441 | GGATCCAGACGAGCGGATAATGCC (EcoRI) | virB11 downstream |
| KSAC707-F | GGATCCTAACCAATTCTGATTAGA (BamHI) | Kan cassette |
| KSAC1638-R | GATATCCGTTGTGTCTCAAAATCTCTG (EcoRV) | Kan cassette |
| SacB-F-SpeI | ACTAGTGCTGGCTTAACTATGCGGCA (SpeI) | sacB |
| SacB-R-SpeI | ACTAGTTCAAACAGGAGGGCTGGAAG (SpeI) | sacB |
| VirB-P-F2 | CTCGAGATGACAGGCATATTTCAA (XhoI) | virB promoter |
| VirB-P-R | CTGCAGGTCTCCTTCTCAGAGAAT (PstI) | virB promoter |
| VirB3-F2 | CTGCAGATGACAACGGCACCACAGGGA (PstI) | virB3 complementation |
| VirB3-R2 | ACTAGTTTATGATTCTCTTTTGCG (SpeI) | virB3 complementation |
| VirB4-F2 | CTGCAGATGGGCGCTCAATCCAAATAC (PstI) | virB4 complementation |
| VirB4-R2 | ACTAGTTCACCTTCCTGTTGATTT (SpeI) | virB4 complementation |
| VirB5-F2 | CTGCAGATGAAGAAGATAATTCTCAGC (PstI) | virB5 complementation |
| VirB5-R2 | ACTAGTTTAATAGGCGGCTTCCAG (SpeI) | virB5 complementation |
| VirB6-F2 | CTGCAGATGGTTAATCCTGTAATCTTT (PstI) | virB6 complementation |
| VirB6-R2 | ACTAGTCTAATCCCTGTTGAACTG (SpeI) | virB6 complementation |
| VirB7-F2 | CTGCAGATGAAAAAGGTAATCCTTGCT (PstI) | virB7 complementation |
| VirB7-R2 | ACTAGTTTAGTCCTCGTAAGTGTC (SpeI) | virB7 complementation |
| VirB8-F2 | CTGCAGATGTTTGGACGCAAACAATCT (PstI) | virB8 complementation |
| VirB8-R2 | ACTAGTTCATTGCACCACTCCCAT (SpeI) | virB8 complementation |
| VirB9-F2 | CTGCAGATGAAAAGATTCCTGCTTGCG (PstI) | virB9 complementation |
| VirB9-R2 | ACTAGTTCATTGCAGGTTCTCCCC (SpeI) | virB9 complementation |
| VirB10-F2 | CTGCAGATGACACAGGAAAACATTCCG (PstI) | virB10 complementation |
| VirB10-R2 | ACTAGTTCACTTCGGTTTGACATC (SpeI) | virB10 complementation |
| VirB11-F2 | CTGCAGATGTCAAACCGAAGTGAC (PstI) | virB11 complementation |
| VirB11-R2 | ACTAGTTCATTGCGTCTTCTCACT (SpeI) | virB11 complementation |
| RTgyrA-F | TGATGCCCTCGTGCGTATG | RT-PCR |
| RTgyrA-R | TTCCGTGACCTTTTCCAGACG | RT-PCR |
| RTvirB-F | TGCCATTCCTTGTCCTCGC | RT-PCR |
| RTvirB-R | CCCACCAACGACGCCTATTG | RT-PCR |
| RTvirB6-F | TATGATTGCCACGACTGCTGTC | RT-PCR |
| RTvirB6-R | ATGACGAGTTGCGAAAACGG | RT-PCR |
| RTvirB11F | CAGGCAAGACCACACTGATGAAAG | RT-PCR |
| RTvirB11R | CGCTTCGCTCGGATAAAACAG | RT-PCR |
a
Nucleotides in primers are numbered according to position in the B. abortus virB locus as described under GenBank accession number AF226278 (56).