FIG. 4.

Cytochromes c and Pro141 and Glu80 mutant variants of ResA in membranes of different strains. (A) SDS-PAGE and heme staining of membranes from B. subtilis 1A1, LUL9, LUN1, LUN5, LUN6, and LUN7. Bands due to the c-type cytochromes of B. subtilis are indicated on the left. We noted that the band due to QcrB (the cytochrome b subunit of the bc complex) is missing from each lane; the reason for this is not clear, but we have routinely observed variable staining of this protein, which contains an unusual covalently attached heme (46). (B) Western blot of membranes from the strains shown in panel A, probed with anti-ResA antiserum.