TABLE 2.
traI deletion mutants of pTiC58 require exogenous quormone for conjugative transfer
| Ti plasmid | traI allele | Transfer frequencya
|
|
|---|---|---|---|
| With quormone | Without quormoneb | ||
| pTiC58accR | Wild type | (3.75 ± 1.75) × 10−3 | NA |
| ΔtraI | <10−8 | (2.05 ± 0.25) × 10−3 | |
| pCMA1 | Wild type | (5.25 ± 2.15) × 10−4 | NA |
| ΔtraI | <10−8 | (3.05 ± 1.25) × 10−4 | |
| pKMA1 | Wild type | (2.65 ± 0.75) × 10−2 | NA |
| ΔtraI | <10−8 | (2.00 ± 0.7) × 10−2 | |
a
Expressed as numbers of transconjugants recovered per input donor in matings with strain C58C1RS as a recipient. Values are the averages for two independent experiments.
b
N-(3-Oxo-octanoyl)-l-homoserine lactone was added at a final concentration of 25 nM as indicated. NA, not applicable (strains with the wild-type allele of traI synthesize their own quormone).