FIG. 2.

Lud135, a temperature-sensitive yghB yqjA mutant. (A) Lud135 transformed with vector pACYC184 (Lud135−, black circles), p-yghB (Lud135+, black diamonds), or p-yqjA (Lud135+, white circles) was grown at 30°C to an OD₆₀₀ of ∼1.0 in LB-chloramphenicol and then diluted 10-fold into prewarmed medium (44°C); growth was subsequently monitored at 15-min intervals. Cultures were diluted 10-fold into fresh prewarmed medium when the culture density reached an OD₆₀₀ of 0.3 to 0.4 to maintain logarithmic growth. Cumulative growth was calculated by correcting for dilution. (B) Growth of Lud135/pACYC184 (labeled “−”), Lud135/p-yqjA, Lud135/p-yghB, Lud135/p-dedA, Lud135/p-yohD, and Lud135/p-yabI on LB-chloramphenicol agar plates at 30 and 42°C.