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SEM of W3110A, Lud135, and BC201. W3110A (A), Lud135 (B), and BC201 (C and D) cells were grown at 30°C in liquid LB medium to mid-log phase, treated as described in Materials and Methods, and imaged with a Cambridge S-260 SEM. Lud135/BC201 shows cell division defects, including the formation of chains of incompletely divided cells and signs of filamentation and membrane bulging (arrows).
