TABLE 1.

PCR primers used in this study

E. coli gene	Primer sequence (forward; reverse)a
yghB	5′-CGCGCATATGGCTGTTATTCAAGATATCATCGCTG-3′ (NdeI); 5′-CGCGAAGCTTGGGGAAAATCGTCAGGCGTTACAG-3′ (HindIII)
yqjA	5′-GCGCCATATGGAACTTTTGACCCAATTGCTGCAAGCC-3′ (NdeI); 5′-GCGCAAGCTTGCGAAGCGACATGCGAGGTATTTGCATC-3′ (HindIII)
dedA	5′-GCGCTCTAGAGTAGTTACAGCCTGAAAGATGACGAGTAC-3′ (XbaI); 5′-GCGCAAGCTTGAACCGCGGAGTTACTTTTTATTTTGCG-3′ (HindIII)
yabI	5′-GCGCCATATGGGCACATTATGCAAGCATTGCTGGAAC-3′ (NdeI); 5′-GCGCAAGCTTTCCTAAACCCCAACCACTTTACGC-3′ (HindIII)
yohD	5′-GCGCCATATGGACTGGCCTATGGATCTCAATACACTTATC-3′ (NdeI); 5′-GCGCAAGCTTCAATGGTTTTACGCCTGATGATCCGG-3′ (HindIII)

^aUnderlining for each primer sequence indicates the site for the restriction endonuclease (given in parentheses) for that sequence.