TABLE 2.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype and descriptiona | Source or reference |
|---|---|---|
| E. coli strains | ||
| W3110 | Wild type, F−, λ− | E. coli genetic stock center, Yale University |
| W3110A | aroA::Tn10 (Tetr P1vir transductant of W3110; LCB273 donor) | 15 |
| Lud135 | W3110A (temp-sensitive mutant), yghB1(G203D), yqjA1(W92TGA) | This work |
| JW3066 | Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− ΔyqjA785::Kanrrph-1 Δ(rhaD-rhaB)568 hsdR514 | 1 |
| JW2975 | Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− ΔmetC790::Kanrrph-1 Δ(rhaD-rhaB)568 hsdR514 | 1 |
| JW2976 | Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− ΔyghB781::Kanrrph-1 Δ(rhaD-rhaB)568 hsdR514 | 1 |
| JW3065 | Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− ΔexuR784::Kanrrph-1 Δ(rhaD-rhaB)568 hsdR514 | 1 |
| BC201 | JW2976, ΔyqjA::Tetr | This work |
| BC202 | W3110, ΔyqjA::Tetr ΔyghB781::Kanr | This work |
| BC203 | W3110, ΔyqjA::Tetr | This work |
| BC204 | W3110, ΔyghB781::Kanr | This work |
| Plasmids | ||
| pET23a | Expression vector; T7 promoter; Ampr | Novagen |
| pET23-yghB | pET23 containing E. coli yghB (NdeI/HindIII sites) | This work |
| pET23-yqjA | pET23 containing E. coli yqjA (NdeI/HindIII sites) | This work |
| pET23-yabI | pET23 containing E. coli yabI (NdeI/HindIII sites) | This work |
| pET23-yohD | pET23 containing yohD (NdeI/HindIII sites) | This work |
| pACYC184 | Low-copy-number vector; Tetr Camr | New England Biolabs |
| p-yghB | XbaI/HindIII fragment of pET23-yghB cloned into pACYC184 | This work |
| p-yqjA | XbaI/HindIII fragment of pET23-yqjA cloned into pACYC184 | This work |
| p-dedA | pACYC184 containing E. coli dedA (XbaI/HindIII sites) | This work |
| p-yabI | XbaI/HindIII fragment of pET23-yabI cloned into pACYC184 | This work |
| p-yohD | XbaI/HindIII fragment of pET23-yohD cloned into pACYC184 | This work |
a
Ampr, ampicillin resistance; Camr, chloramphenicol resistance.