TABLE 3.
Visible colony formation of Lud135 and BC201 when grown on LB media containing different supplements
| LB medium supplement (concn in mM)a | Colony formation at indicated temp seen forb:
|
|||
|---|---|---|---|---|
| Lud135
|
BC201
|
|||
| 30°C | 42°C | 30°C | 42°C | |
| None | + | − | + | − |
| Ca2+ (10) | + | + | + | + |
| Sr2+ (10) | + | + | + | + |
| Mg2+ (10) | + | + | + | + |
| Ba2+ (5) | + | + | + | + |
| NaCl (0) | − | − | − | − |
| NaCl (100) | − | − | + | − |
| NaCl (170)c | + | − | + | − |
| NaCl (200) | + | − | + | −/+ |
| NaCl (300) | + | + | + | + |
| NaCl (400) | + | + | + | + |
| NaCl (500) | + | + | + | + |
| NaCl (600) | + | − | + | −/+ |
| Sucrose (400) | + | − | + | − |
a
Calcium, magnesium, strontium, barium, sodium chloride, or sucrose was included in LB agar plates at the indicated concentrations.
b
Lud135 or BC201 (∼500 CFU) was plated and incubated at 30 and 42°C overnight. Parent strain W3110A grew under all conditions (not shown). A plus sign (+) indicates clearly visible colony formation, a minus sign (−) indicates no visible colony formation, and minus/plus (−/+) indicates barely visible colony formation after the overnight incubation.
c
LB normally contains ∼170 mM NaCl (10 g/liter).