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FlhDC controls regulatory genes for exoprotein production and motility. (A) Northern blot analysis of gacA, rsmA, rsmC, hexA, and fliA in Ecc71 (lane 1) and its FlhDC- mutant AC5140 (lane 2). Each lane contained 15 μg of total RNA. The arrow shows the levels of total RNA as revealed by ethidium bromide staining of denatured agarose gel. (B and C) β-Galactosidase activities of transcriptional gacA-lacZ fusion pAKC1243 and rsmC-lacZ fusion pAKC1244 in AC5006 and AC5141, respectively.
