FIG. 2.

HPLC separation of B. anthracis spore muropeptides. Unless otherwise noted, PG preparations were digested with muramidase, reduced, and separated as previously described (19). (A) Muropeptides derived from dormant B. subtilis spores. Peaks are numbered as in reference 19. Early-eluting peaks labeled “b” are buffer components present in blank samples. (B) Muropeptides derived from dormant B. anthracis spores. Peaks are designated as in Table 1. (C) Muropeptides derived from B. anthracis spores 15 min after the initiation of germination. (D) Muropeptides derived from the exudate of B. anthracis spores 15 min after the initiation of germination. Peaks labeled “ex” are spore exudate components that, based upon amino acid analysis, are not derived from PG. Peaks are designated as in Table 1; however, the “a” in germination-specific peak designations is omitted due to space limitations. (E) Muropeptides derived from the exudate of B. anthracis spores 15 min after the initiation of germination, but with no muramidase digestion.