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. 2008 May 2;190(13):4541–4548. doi: 10.1128/JB.00249-08

FIG. 3.

FIG. 3.

Kinetics of B. anthracis and B. subtilis spore germination. Purified spores were heat activated and stimulated to germinate by addition of l-alanine and inosine at time zero. Samples were removed for measurement of the optical density, DPA release, and PG release at the indicated times. (A) Decrease in optical density (open symbols) and release of DPA (filled symbols) were determined for B. anthracis (squares) and B. subtilis (circles). (B) Release of Dpm (open symbols) and muramic acid (filled symbols) was determined for B. anthracis (squares) and B. subtilis (circles). Data from a single experiment are shown; however, similar results were obtained in two additional experiments.