FIG. 4.

Muramidase cleaves the glucosaminidase product muropeptide aG6 to produce muropeptide aG4. Muropeptide aG6 was purified using HPLC separation as shown in Fig. 2 and was then desalted and further purified by HPLC using an acetonitrile/trifluoroacetic acid buffer system. The purified muropeptide was incubated in 12.5 mM NaPO₄ (pH 5.5) without (A) and with (B) mutanolysin, followed by reduction and HPLC separation as shown in Fig. 2.