FIG. 1.
RscS-mediated attachment to a glass surface in RR mutants. (A) Wild-type (WT) and RR mutant strains of V. fischeri carrying either RscS overexpression vector pKG11 (+) or the vector control, pKV69 (−), were grown statically in HMM containing glucose and Casamino Acids and stained with CV to visualize surface-attached material (representative photographs from an experiment conducted in triplicate). (B) Stain was removed by agitation with 1-mm glass beads and ethanol and quantitated by spectrophotometry. Induction represents the OD600 of a given strain carrying pKG11 divided by that of the same strain carrying the vector control.