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RscS-mediated pellicle formation in RR mutants. Wild-type (WT) and RR mutant strains of V. fischeri carrying either pKG11 or a vector control (pKV69) were grown statically in HMM containing glucose and Casamino Acids for 48 h (A and B, as indicated) or 72 h (B). Pellicle formation was assessed by dragging a sterile toothpick through the culture surface and scored as described in Materials and Methods. Photographs are representative of samples from experiments conducted in triplicate.
