TABLE 5.
Optimal conditions described in this study to perform phenotypic detection of MBL production among P. aeruginosa, Acinetobacter spp., and Enterobacteriaceae clinical isolates
| Isolate group | Test | IMBL | β-Lactam substrate | Expl conditiona |
|---|---|---|---|---|
| Acinetobacter spp. | DDST | 5 μl MPA (1.4 mM) | Imipenem | Distance, 2.0 cm |
| P. aeruginosa | DDST | 5 μl MPA (1.4 mM) | Ceftazidime | Distance, 2.0 cm |
| Enterobacteriaceae | CD | 10 μl EDTA (100 mM) | Imipenem | Breakpoint, 5 mm |
a
Breakpoint is the increase in the size of inhibition zone.