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Evaluation of analytical sensitivity with serial dilutions of 28S rDNA of Aspergillus fumigatus
| Copies/μla | No. PCR-positive samples/total | % Positiveb |
|---|---|---|
| 110 | 24/24 | 100 |
| 55 | 24/24 | 100 |
| 28 | 23/24 | 99 |
| 14 | 22/24 | 94 |
| 7 | 19/24 | 76 |
| 4 | 17/24 | 64 |
| 2 | 14/24 | 55 |
| 1 | 10/24 | 50 |
Serial dilution of 28S rDNA copies of Aspergillus fumigatus amplified with the NL1/NL4 primer system (21).
Probability of a positive PCR signal calculated by probit analysis.
