TABLE 3.
Quantitation of HIV-1 RNA and DNA in PBMC samples by seminested and single-step real-time PCR methods
| Sample code | Mean copy no. in reaction ± SDa
|
|||||
|---|---|---|---|---|---|---|
| usRNA
|
msRNA
|
prDNA
|
||||
| Seminested real-time PCR (n = 8) | Single-step real-time PCR (n = 1) | Seminested real-time PCR (n = 5) | Single-step real-time PCR (n = 1) | Seminested real-time PCR (n = 12) | Single-step real-time PCR (n = 2) | |
| 1 | ND | ND | 11.96 ± 0.35 | ND | ND | ND |
| 2 | ND | ND | ND | ND | 8.68 ± 1.34 | ND |
| 3 | 10.89 ± 0.13 | ND | 9.44 ± 0.16 | ND | 10.46 ± 1.87 | ND |
| 4 | 19.76 ± 14.17 | ND | 12.11 ± 0.55 | ND | 8.21 ± 1.00 | ND |
| 5 | 6.58 ± 2.50 | ND | ND | ND | 6.23 ± 0.33 | ND |
| 6 | ND | ND | ND | ND | ND | ND |
| 7 | 86.63 ± 5.87 | ND | ND | ND | 49.41 ± 14.42 | ND |
| 8 | ND | ND | ND | ND | 29.89 ± 4.99 | ND |
| 9 | 197.58 ± 38.27 | ND | ND | ND | 45.41 ± 0.91 | ND |
| 10 | 21.44 ± 0.31 | ND | ND | ND | 10.66 ± 5.18 | ND |
| 11 | ND | ND | ND | ND | 7.37 ± 0.89 | ND |
| 12 | 30.26 ± 14.32 | ND | 24.65 ± 2.09 | ND | 16.33 ± 0.05 | ND |
| 13 | ND | ND | ND | ND | 84.86 ± 0.70 | 2,547.61 ± 2,292.91 |
| 14 | 859.74 ± 110.51 | 1,789.60 ± 1,523.18 | 12.84 ± 1.18 | 156.90 ± 28.69 | 156.45 ± 12.06 | 3,734.30 ± 3,424.77 |
a
n, Number of samples detected. ND, not detected. The mean percent coefficients of variation (95% confidence intervals [where applicable]) were as follows: usRNA seminested real-time PCR, 25% (7% to 43%); usRNA single-step real-time PCR, 85%; msRNA seminested real-time PCR, 5% (2% to 8%); msRNA single-step real-time PCR, 18%; and prDNA seminested real-time PCR, 14% (6% to 22%); prDNA single-step real-time PCR, 91% (89% to 93%).