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. 1999 Dec 7;96(25):14523–14528. doi: 10.1073/pnas.96.25.14523

Figure 2.

Figure 2

Gene expression of STEAP in tissues and cell lines. (A) Human normal tissue filters contain 2 μg of mRNA per lane. Lanes are: 1, heart; 2, brain; 3, placenta; 4, lung; 5, liver; 6, skeletal muscle; 7, kidney; 8, pancreas; 9, spleen; 10, thymus; 11, prostate; 12, testis; 13, ovary; 14, small intestine; 15, colon; and 16, peripheral blood leukocytes. (B) Xenograft and cell-line filters were prepared with 10 μg of total RNA per lane. Lanes are: 1, primary prostate epithelial cells; 2, prostate; 3, LAPC-4 AD; 4, LAPC-4 AI; 5, LAPC-9 AD; 6, LAPC-9 AI; 7, LNCaP; 8, PC-3; 9, DU145; 10, PANC-1; 11, BxPC-3; 12, HPAC; 13, Capan-1; 14, CACO-2; 15, LOVO; 16, T84; 17, COLO-205; 18, KCL-22 (acute lymphocytic leukemia, ALL); 19, HT1197; 20, SCABER; 21, UM-UC-3; 22, TCCSUP; 23, J82; 24, 5637; 25, RD-ES (Ewing sarcoma, EWS); 26, CAMA-1; 27, DU4475; 28, MCF-7; 29, MDA-MB-435s; 30, NTERA-2; 31, NCCIT; 32, TERA-1; 33, TERA-2; 34, A431; 35, HeLa; 36, OV-1063; 37, PA-1; 38, SW 626; and 39, CAOV-3. The blots were probed by using an SSH-derived gene fragment. All RNA samples were normalized by ethidium bromide staining and subsequent analysis with a β-actin probe.