TABLE 3.
Comparison of RQ-mPCR results between in pneumococcal positive sputum samples and in S. pneumoniae isolates
| Sample group and gene | No. of samples | Positive sputum samples by RQ-mPCR among samples with disagreement (n = 5)b
|
No. false positive/no. false negative | % Sensitivity | % Specificity | ||||
|---|---|---|---|---|---|---|---|---|---|
| 102/5F01 | 107/5F23 | 113/5F28 | 101/6S11 | 101/6D13 | |||||
| Samples with complete agreement | 51/56a | ||||||||
| Samples with disagreement | |||||||||
| lytA | 51 | + | + | + | + | + | 0/0 | 100 | 99.3 |
| erm(B) | 27 | − | − | + | FP | FP | 2/0 | 100 | 93.9 |
| mef(A) | 19 | FP | + | FP | FP | FP | 2/0 | 100 | 94.8 |
| pbp1a | 15 | FN | − | FP | + | + | 1/1 | 94.4 | 97.5 |
| pbp2x | 24 | + | FP | FP | + | + | 2/0 | 100 | 94.1 |
| pbp2b | 12 | + | FP | FP | + | + | 2/0 | 100 | 95.6 |
That is, the number of samples in complete agreement with RQ-mPCR results in S. pneumoniae isolates/number of all samples.
FP, false-positive; FN, false-negative.